Abstract

Background - The quality of red blood cells (RBCs) stored in red cell concentrates (RCCs) is influenced by processing, storage and donor characteristics, and can have a clinical impact on transfused patients.To evaluate RBC properties and their potential impact in a transfusion setting, a simple in vitro-transfusional model has been developed.
Materials and methods - Transfusion was simulated by mixing a washed RBC pool from two male-derived RCCs stored at 4°C with a pool of 15 male-derived fresh frozen plasma (FFP) units, representing the recipient, at a hematocrit (HCT) of 30% (“control” setting) or 5% (alternative model).The mixtures were incubated at 37°C, 5% of CO2 up to 48 h.Different metabolites, hemolysis and microvesicles (MVs) were quantified at several incubation times and RBC-morphology changes and deformability after incubation. For each model, biological triplicates have been investigated with RCCs at storage days 2 and 43.
Results - The 5%-HCT model restored the 2,3-DPG level and maintained the ATP level. Furthermore, glucose consumption and corresponding lactate production were increased in the 5%- vs the 30%-HCT condition. Lower hemolysis was observed with 5%-HCT, but only at day 2.However, morphological analysis by digital holographic microscopy (DHM) revealed a decreased fraction of discocytes at 5% rather than at 30% of HCT at storage day 2 but at day 43, the trend was inverted. Concordantly, RBCs incubated at 5% of HCT were more deformable than at 30% at day 43 (p<0.0001).
Discussion - Higher metabolic activity of RBCs in the 5%-HCT condition was promoted by a higher glucose availability and limited cell-waste accumulation.The conditions of the new proposed model thus enabled rejuvenation of RBCs and maintained them in a physiological-close state in contrast to the 30%-HCT model.It may be used as a first approach to evaluate e.g., the impact of donor and recipient characteristics on RBC properties.

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Authors

Emmanuel Längst - Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS SA, Epalinges, Switzerland; Faculté de Biologie et de Médecine, University of Lausanne (UNIL), Lausanne, Switzerland

David Crettaz - Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS SA, Epalinges, Switzerland

Julien Delobel - Laboratoire de Recherche and Unité d’Hématologie-Oncologie Pédiatrique, Service de Pédiatrie, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland

Raffaele Renella - Laboratoire de Recherche and Unité d’Hématologie-Oncologie Pédiatrique, Service de Pédiatrie, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland

Manon Bardyn - Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS SA, Epalinges, Switzerland

Gerardo Turcatti - Biomolecular Screening Facility (BSF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

Jean-Daniel Tissot - Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS SA, Epalinges, Switzerland

Michel Prudent - Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS SA, Epalinges, Switzerland; Faculté de Biologie et de Médecine, University of Lausanne (UNIL), Lausanne, Switzerland; Center for Research and Innovation in Clinical Pharmaceutical Sciences, Institute of Pharmaceutical Sciences of Western Switzerland, Centre Hospitalier Universitaire Vaudois (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva (UNIGE), University of Lausanne (UNIL), Switzerland

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