Original article

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Lipidomic analysis of differently prepared platelet concentrates in additive solution during storage

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Key words: platelet, lipidomic, transfusion
Publication Date: 2022-11-04

Abstract

Background - Structural and biochemical changes in stored platelets are influenced by collection and processing methods. Lesions may appear during platelet concentrate storage, some of which may be involved in adverse transfusion reactions. The preparation and storage of platelet concentrates (PC) may modify and even damage the lipid mediator content. The aim of this study was to investigate the lipidomic profile identified in the supernatants of PCs according to processing and storage conditions, both after leukocyte filtration and contained in platelet additive solution (PAS), comparing single donor apheresis (SDA) products with pooled buffy coat (BC) products.
Materials and methods - We investigated the accumulation of various lipid mediators including lysophospholipids (LP) and eicosanoids in SDA and BC products stored for 0-5 days. All products were processed following  French Blood Establishment (EFS) procedures in accordance with
EDQM/GTS European Standards. Both SDA and BC were leukocyte reduced and conserved in 35% autologous donor plasma and 65% platelet additive solution. Lipidomic analysis was performed on PC supernatants using LS/MS spectrometry.
Results - Our data demonstrate that lysophosphatidylcholine (LPC) levels were higher in BCs compared to SDAs, with no difference in lysophosphatidic acid (LPA) expression between the two preparation methods. Results for other eicosanoids showed greater similarity; indeed, no clear pattern emerged from analysis of eicosanoids in terms of storage time and process. In general, we observed longitudinal lipid mediator modulation for both SDAs and BCs, particularly at later time points.
Discussion - The expression of LPC and some eicosanoids in BCs could be used as novel biomarkers of PC quality. Future studies are needed to explore their impact on adverse transfusion reactions.

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Authors

Anne-Claire Duchez - Etablissement Français du Sang Auvergne-Rhône-Alpes, Saint-Étienne, France; University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

Sébastien Fauteux-Daniel - Etablissement Français du Sang Auvergne-Rhône-Alpes, Saint-Étienne, France; University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

Theo Ebermeyer - University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

Marco Heestermans - Etablissement Français du Sang Auvergne-Rhône-Alpes, Saint-Étienne, France; University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

Charles-Antoine Arthaud - Etablissement Français du Sang Auvergne-Rhône-Alpes, Saint-Étienne, France; University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

Marie-Ange Eyraud - Etablissement Français du Sang Auvergne-Rhône-Alpes, Saint-Étienne, France; University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

Amélie Prier - Etablissement Français du Sang Auvergne-Rhône-Alpes, Saint-Étienne, France; University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

Estelle Audoux - Etablissement Français du Sang Auvergne-Rhône-Alpes, Saint-Étienne, France; University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

Jean-Charles Portais - MetaToul-Lipidomic MetaboHUB Core Facility, Inserm, U1048, Toulouse France; Laboratoire d'Hématologie, Centre Hospitalier Universitaire de Toulouse, Toulouse, France

Justine Bertrand-Michel - MetaToul-Lipidomic MetaboHUB Core Facility, Inserm, U1048, Toulouse France; INSERM UMR 1214, ToNIC: Toulouse NeuroImaging Center, Toulouse, France

Olivier Garraud - Etablissement Français du Sang Auvergne-Rhône-Alpes, Saint-Étienne, France; University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

Hind Hamzeh-Cognasse - University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

Eric Boilard - Department of Infectious Diseases and Immunity, Centre de Recherche du CHU de Québec, Canada; 7Université Laval and Centre de recherche ARThrite, Québec, Canada

Fabrice Cognasse - Etablissement Français du Sang Auvergne-Rhône-Alpes, Saint-Étienne, France; University of Jean Monnet, Mines Saint-Étienne, INSERM, U 1059 SAINBIOSE, Saint-Étienne, France

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