Abstract

Background. Early detection of the platelet storage lesion is still a challenge in transfusion practice. Using flow cytometry, we evaluated the appearance of the storage lesion, based on the expression of platelet activation markers, in total platelets and platelet populations.
Materials and methods. Buffy-coat-derived platelet concentrates were stored under standard conditions for 5 days. The expression of activation antigens CD42b, CD36, CD62p and phosphatidylserine on total platelets and populations of small, medium-sized and large platelets was analysed by flow cytometry on storage days 1, 3 and 5.
Results. The activation/lesion on total platelets and each platelet population was detected on storage day 3, by the increased expression of CD36. On the same day, increased expression of CD42b and CD62p was detected, but only on large platelets. Small and medium-sized platelets had increased CD62p expression only on day 5. Externalisation of phosphatidylserine was not detected.
Discussion. Evaluation of the level of expression of various activation markers on different platelet populations could be an additional valid analysis in cell quality control of platelet concentrates, and in the assessment of novel approaches to platelet concentrate manipulation.

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Authors

Dušan Vučetić - Institute for Transfusiology and Haemobiology, Military Medical Academy; Faculty of Medicine of Military Medical Academy, University of Defence

Vesna Ilić - Institute for Medical Research, University of Belgrade

Danilo Vojvodić - Faculty of Medicine of Military Medical Academy, University of Defence; Institute of Medical Research, Military Medical Academy

Vesna Subota - Faculty of Medicine of Military Medical Academy, University of Defence; Institute of Medical Biochemistry, Military Medical Academy

Milena Todorović - Haematology Clinic of the Clinical Centre of Serbia

Bela Balint - Institute for Transfusiology and Haemobiology, Military Medical Academy; Faculty of Medicine of Military Medical Academy, University of Defence; Institute for Medical Research, University of Belgrade; Serbian Academy of Sciences and Arts, Belgrade, Serbia

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