Original article

Blood Transfusion 2-2018 (March - April)

Prevalence of antibodies to a new histo-blood system: the FORS system

Authors

Key words: FORS blood group, human anti-Forssman, function-spacer-lipids constructs, ABO blood-group prevalence
Publication Date: 2016-10-24

Abstract

Background. In 1987, three unrelated English families were reported with a putative blood subgroup called Apae. Swedish researchers later found evidence leading to abolishment of the Apae subgroup and establishment instead of the FORS blood group system (System 31 - ISBT, 2012). It is important to know the prevalence of antibodies in order to make the best decisions in transfusion medicine. Cells expressing the Forssman saccharide, such as sheep erythrocytes, are needed to detect the anti-Forssman antibody. The aim of this study was to define the prevalence of human anti-Forssman antibody.
Materials and methods. Plasma samples from 800 individuals were studied. Sheep erythrocytes or Forssman "kodecytes" were mixed with the plasma samples using the tube technique. Plasma from an Apae individual was used as a negative control and monoclonal anti-Forssman antibody (M1/22.25.8HL cell line supernatant) was used as the positive control.
Results. Of the 800 individuals tested, one was negative for the presence of anti-Forssman antibody. We compared the anti-Forssman antibody reaction pattern between genders and found that males have weaker reactions than females, both at room temperature (p=0.026) and at 37 °C (p=0.043). We also investigated the reaction pattern of anti-Forssman antibody in relation to ABO and Rh blood group types without finding any significant differences.
Discussion. Sheep erythrocytes are suitable for searching for human anti-Forssman antibody. The quantity of anti-Forssman antibodies in plasma is higher in females than in males. In the population (n=800) studied here, we found one individual lacking the anti-Forssman antibody. These results contribute to the data already published, confirming that FORS is a rare blood group.

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Authors

Carlos Jesus - Biomedical Sciences Department of ESTESC - Coimbra Health School, Polytechnic Institute of Coimbra, Coimbra, Portugal

Camilla Hesse - Department for Clinical Chemistry and Transfusion Medicine, Institute for Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden

Clara Rocha - Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Department Complementary Sciences, Coimbra; INESC Coimbra, Coimbra

Nádia Osório - Biomedical Sciences Department of ESTESC - Coimbra Health School, Polytechnic Institute of Coimbra, Coimbra, Portugal

Ana Valado - Biomedical Sciences Department of ESTESC - Coimbra Health School, Polytechnic Institute of Coimbra, Coimbra, Portugal

Armando Caseiro - Biomedical Sciences Department of ESTESC - Coimbra Health School, Polytechnic Institute of Coimbra, Coimbra, Portugal; I&D Unit "Química-Física Molecular", Faculty of Science and Technology, University of Coimbra, Coimbra, Portugal

António Gabriel - Biomedical Sciences Department of ESTESC - Coimbra Health School, Polytechnic Institute of Coimbra, Coimbra, Portugal

Lola Svensson - Department for Clinical Chemistry and Transfusion Medicine, Institute for Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden

Ali-Reza Moslemi - Department of Pathology, Institute for Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden

Wafa Abu Siba - Department of Medical Laboratory Sciences, Faculty of Health Professions, Al-Quds University, East Jerusalem, Palestine

Mahmoud A. Srour - Department of Medical Laboratory Sciences, Faculty of Health Professions, Al-Quds University, East Jerusalem, Palestine

Cristina Pereira - Blood and Transfusion Medicine Department, Coimbra Hospital and University Centre (CHUC), Coimbra

Jorge Tomaz - Blood and Transfusion Medicine Department, Coimbra Hospital and University Centre (CHUC), Coimbra

Paulo Teixeira - Biomedical Sciences Department of ESTESC - Coimbra Health School, Polytechnic Institute of Coimbra, Coimbra, Portugal; Department of Anatomic Pathology, Coimbra Hospital and University Centre (CHUC), Coimbra

Fernando Mendes - Biophysics and Biomathematics Institute, IBILI-Faculty of Medicine, University of Coimbra, Coimbra; CIMAGO, FMUC-Faculty of Medicine, University of Coimbra, Coimbra; CNC.IBILI, University of Coimbra, Coimbra, Portugal

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