Original article

Blood Transfusion - 2 2022 (March-April)

Increased platelet thrombus formation under flow conditions in whole blood from polycythaemia vera patients

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Key words: polycythaemia vera, thrombosis, platelet adhesiveness, haematocrit, erythrocytes
Publication Date: 2021-03-30

Abstract

Background - Polycythaemia vera is a myeloproliferative neoplasm characterised by a high incidence of thrombosis. The contribution of platelets, key players in haemostasis, in this setting is still unclear. So far, the majority of studies have been focussed on specific platelet abnormalities but not on their actual capacity to form thrombi. The aim of this study was to characterise, ex vivo under flow conditions, the capacity of platelets from patients with polycythaemia vera to adhere to collagen and induce thrombus formation.
Materials and methods - Thirty-nine patients and 30 healthy controls were studied. Thrombus formation was induced by perfusing whole blood over a collagen-coated surface, in a parallel-plate flow chamber coupled to a fluorescent microscope. This dynamic system enables platelet adhesion and thrombus formation to be followed in real time and also allows measurements of the extent of the thrombus and platelet surface antigen expression. Laboratory data were analysed in the light of the patients’ main haematological parameters and therapies.
Results - Platelet adhesion was significantly greater in patients than in control subjects. Patient thrombi were usually larger and more complex than those formed by control platelets. A significant positive correlation was found between platelet adhesion and both the haematocrit and red blood cell count. These parameters remained significantly correlated with platelet adhesion also after multivariable analysis adjusted for gender, age, therapy and JAK2V617F allele burden. Furthermore, subjects with a haematocrit >45% had significantly greater platelet adhesion than subjects with a haematocrit <45%.
Discussion - Our data indicate that increased platelet adhesion participates in the thrombotic diathesis of patients with polycythaemia vera, and that the haematocrit level can affect the adhesive and thrombus forming capacities of platelets.

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Authors

Alfonso Vignoli - Department of Immunohaematology and Transfusion Medicine, “Papa Giovanni XXIII” Hospital, Bergamo, Italy

Sara Gamba - Department of Immunohaematology and Transfusion Medicine, “Papa Giovanni XXIII” Hospital, Bergamo, Italy

Paola E. J. van der Meijden - Department of Biochemistry,CARIM, Maastricht University, Maastricht, the Netherlands

Marina Marchetti - Department of Immunohaematology and Transfusion Medicine, “Papa Giovanni XXIII” Hospital, Bergamo, Italy

Laura Russo - Department of Immunohaematology and Transfusion Medicine, “Papa Giovanni XXIII” Hospital, Bergamo, Italy

Serena Tessarolo - Department of Immunohaematology and Transfusion Medicine, “Papa Giovanni XXIII” Hospital, Bergamo, Italy

Cinzia Giaccherini - Department of Immunohaematology and Transfusion Medicine, “Papa Giovanni XXIII” Hospital, Bergamo, Italy

Frauke Swieringa - Department of Biochemistry,CARIM, Maastricht University, Maastricht, the Netherlands

Hugo ten Cate - Department of Internal Medicine, CARIM, Maastricht University, Maastricht, the Netherlands

Guido Finazzi - Department of Haematology, “Papa Giovanni XXIII” Hospital, Bergamo, Italy

Johan W.M. Heemskerk - Department of Biochemistry,CARIM, Maastricht University, Maastricht, the Netherlands

Anna Falanga - Department of Immunohaematology and Transfusion Medicine, “Papa Giovanni XXIII” Hospital, Bergamo, Italy; School of Medicine and Surgery, University of Milan-Bicocca, Monza, Italy

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