Original article

Blood Transfusion - 1 2020 (January-February)

Cigarette smoking and antioxidant defences in packed red blood cells prior to storage

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Key words: blood donation, cotinine, oxidative stress, tobacco, vitamins
Publication Date: 2019-11-20

Abstract

Background. Red blood cells from smoking donors can have more lesions from oxidative stress, decreasing the benefits of blood transfusion. We aimed to explore the effect of cigarette smoking on the oxidative status of packed red blood cells (PRBCs) prior to storage.
Materials and methods. We compared serum vitamin C, plasmatic malondialdehyde (MDA), and non-protein thiol groups (GSH) levels in PRBCs, as well glutathione peroxidase (GPx) and glutathione s-transferase (GST) activity in PRBCs from smoking (n=36) and non-smoking (n=36) donors. We also correlated urinary cotinine levels with these parameters.
Results. Cigarette smoking was associated with decreased serum levels of vitamin C and GPx, and increased GST activity in PRBCs. We found negative correlations between cotinine, GPx activity and vitamin C levels, and a positive correlation between cotinine and GST activity.
Discussion. Cigarette smoking changed antioxidant defences of PRBCs prior to storage and these parameters are correlated with cotinine levels. Increased RBC antioxidants such as GST may reflect an exposure to oxidants during erythropoiesis. Because of the inability of mature RBCs to resynthesise antioxidants, PRBCs from smokers may have higher risk of storage lesions than those from non-smoker donors.

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Authors

Renata E. Boehm - Post-graduate Progamme in Pharmacology and Therapeutics, Universidade Federal do Rio Grande do Sul (UFRGS); Haemotherapy Unit of the Hospital de Clínicas de Porto Alegre (HCPA)

Sabrina N. Do Nascimento - Toxicology Laboratory, Department of Analyses, (LATOX), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

Carolina R. Cohen - Haemotherapy Unit of the Hospital de Clínicas de Porto Alegre (HCPA)

Solange Bandiera - Post-graduate Progamme in Pharmacology and Therapeutics, Universidade Federal do Rio Grande do Sul (UFRGS)

Rianne R. Pulcinelli - Post-graduate Progamme in Pharmacology and Therapeutics, Universidade Federal do Rio Grande do Sul (UFRGS)

Almeri M. Balsan - Haemotherapy Unit of the Hospital de Clínicas de Porto Alegre (HCPA)

Nuryan S. Fao - Toxicology Laboratory, Department of Analyses, (LATOX), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

Caroline Peruzzi - Toxicology Laboratory, Department of Analyses, (LATOX), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

Solange C. Garcia - Toxicology Laboratory, Department of Analyses, (LATOX), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

Leo Sekine - Haemotherapy Unit of the Hospital de Clínicas de Porto Alegre (HCPA)

Tor G.H. Onsten - Haemotherapy Unit of the Hospital de Clínicas de Porto Alegre (HCPA)

Rosane Gomez - Post-graduate Progamme in Pharmacology and Therapeutics, Universidade Federal do Rio Grande do Sul (UFRGS)

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