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PLASTIC RECONSTRUCTIVE AND REGENERATIVE SURGERY

Abstract

We have observed significant variation in the volume of eluate from the process of DNA extraction. Such variation may affect the precision of downstream, quantitative DNA measurements. Therefore, we investigated this eluate variation and assessed the potential correlation between DNA concentration and eluate volume in repetitive testing of pooled human plasma sample material. The mean eluate volume of expected 60 µL eluate was 63.3 µL with a coefficient of variation of 6.6%. However, although the total amount of DNA varied and correlated with eluate volume (Spearman rank correlation: r = 0.67), the DNA concentration remained unchanged (p=0.0991), (Spearman rank correlation: r = 0.35). This finding showed that variation in the 60 µL elution volume from the QIAsymphony instrument does not affect downstream, quantitative DNA measurements.

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Authors

Frederik Banch Clausen - Laboratory of Blood Genetics, Department of Clinical Immunology, Copenhagen University Hospital, Copenhagen, Denmark https://orcid.org/0000-0003-2487-5373

Victoria Bølling Jensen - Laboratory of Blood Genetics, Department of Clinical Immunology, Copenhagen University Hospital, Copenhagen, Denmark https://orcid.org/0009-0005-7406-6488

Caroline Lai Kjar - Laboratory of Blood Genetics, Department of Clinical Immunology, Copenhagen University Hospital, Copenhagen, Denmark https://orcid.org/0009-0007-2948-2495

Steffen Ullitz Thorsen - Laboratory of Blood Genetics, Department of Clinical Immunology, Copenhagen University Hospital, Copenhagen, Denmark https://orcid.org/0000-0003-4783-2931

Leif Kofoed Nielsen - Department of Technology, Faculty of Health and Technology, University College Copenhagen, Copenhagen, Denmark https://orcid.org/0000-0002-6956-1821

Grethe Risum Krog - Laboratory of Blood Genetics, Department of Clinical Immunology, Copenhagen University Hospital, Copenhagen, Denmark https://orcid.org/0000-0002-9663-8476

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