Blood Transfusion 2/2017 - Thematic issue on "Red blood cell storage and clinical outcomes: new insights" (March-April)
Omics markers of the red cell storage lesion and metabolic linkage
Authors:  Angelo D'Alessandro, Travis Nemkov, Julie Reisz, Monika Dzieciatkowska, Matthew J. Wither, Kirk C. Hansen
Pages:  137-144
To cite this article:  Blood Transfus 2017; 15: 137-44
Doi:  10.2450/2017.0341-16
Published online:  20/02/2017

The introduction of omics technologies in the field of Transfusion Medicine has significantly advanced our understanding of the red cell storage lesion. While the clinical relevance of such a lesion is still a matter of debate, quantitative and redox proteomics approaches, as well quantitative metabolic flux analysis and metabolic tracing experiments promise to revolutionise our understanding of the role of blood processing strategies, inform the design and testing of novel additives or technologies (such as pathogen reduction), and evaluate the clinical relevance of donor and recipient biological variability with respect to red cell storability and transfusion outcomes. By reviewing existing literature in this rapidly expanding research endeavour, we highlight for the first time a correlation between metabolic markers of the red cell storage age and protein markers of haemolysis. Finally, we introduce the concept of metabolic linkage, i.e. the appreciation of a network of highly correlated small molecule metabolites which results from biochemical constraints of erythrocyte metabolic enzyme activities. For the foreseeable future, red cell studies will advance Transfusion Medicine and haematology by addressing the alteration of metabolic linkage phenotypes in response to stimuli, including, but not limited to, storage additives, enzymopathies (e.g. glucose 6-phosphate dehydrogenase deficiency), hypoxia, sepsis or haemorrhage. 

Keywords: mass spectrometry, advanced omics, Transfusion Medicine, blood, storage.
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