Blood Transfusion - 3 2014 (July - September)
ABO chimerism with a minor allele detected by the peptide nucleic acid-mediated polymerase chain reaction clamping method
Authors:  Rie Sano, Yoichiro Takahashi, Tamiko Nakajima, Mayumi Yoshii, Rieko Kubo, Keiko Takahashi, Yoshihiko Kominato, Haruo Takeshita, Toshihiro Yasuda, Hatsue Tsuneyama, Makoto Uchikawa, Kazumi Isa, Kenichi Ogasawara
Pages:  431-434
To cite this article:  Blood Transfus 2014; 12: 431-4
Doi:  10.2450/2014.0162-13
Published online:  02/01/2014

Discrepancy of results of ABO groups between red cell testing and serum testing is a significant issue in transfusion medicine1. One of its reported causes is blood chimerism, which shows mixed-field agglutination. However, mixed-field agglutination can be seen in various situations, including ABO-incompatible stem cell transplantation, recent transfusions of type-O red blood cells in a non-O recipient, and rare ABO subgroups such as B3 and chimera. Several molecular approaches have been employed to identify chimerism, namely ABO genotyping, human leucocyte antigen typing and DNA short tandem repeat assays2. These methods can typically detect three or more alleles of a chimera. However, some chimeras with a minor allele population might be overlooked because of preferential amplification of the major allele during ordinary polymerase chain reaction (PCR) analysis. (...)
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