Blood Transfusion - 3 2011 (July - September)

Increased detection of clinically significant antibodies and decreased incidence of delayed haemolytic transfusion reaction with the indirect antiglobulin test potentiated by polyethylene glycol compared to albumin: a Japanese study
Authors:  Miho Okutsu, Hitoshi Ohto, Hiroyasu Yasuda, Kinuyo Kawabata, Satoshi Ono, Shunnichi Saito, Akiko Sugawara, Masami Kikuchi, Saori Miura, Youko Ishii, Kazuya Watanabe, Yuriko Tohyama, and Kenneth E. Nollet
Pages:  311-319
To cite this article:  Blood Transfus 2011; 9: 311-9
Doi:  10.2450/2010.0057-10
Published online:  26/11/2010

Background. The indirect antiglobulin test (IAT) can be potentiated by agents such as polyethylene glycol (PEG-IAT) and albumin (Alb-IAT). PEG-IAT is generally regarded as superior to Alb-IAT for the detection of clinically significant red blood cell (RBC) antibodies. However, supporting data come from Caucasian-dominant populations. Non-Caucasian populations should be investigated as well.
Material and methods. In this single-centre, retrospective, sequential study, Alb-IAT was used from 1989 to 1996 (8 years) and PEG-IAT from 1997 to 2008 (12 years). Pre-transfusion RBC alloantibody detection rates and specificity, post-transfusion alloantibody production, and the incidence of delayed haemolytic transfusion reaction were assessed and compared for the two periods.
Results. Although overall RBC alloantibody detection rates were comparable, PEG-IAT more frequently detected clinically significant antibodies such as anti-E, anti-Fyb, and anti-Jka, and less frequently detected insignificant antibodies such as anti-Leb and anti-P1. New alloantibodies emerged comparably during the two periods. Delayed haemolytic transfusion reaction was less frequent during the PEG-IAT period (0.30% versus 0.12%, p<0.05).
Conclusion. PEG-IAT was superior in the detection of clinically significant antibodies, reduced the detection of insignificant antibodies, and prevented delayed haemolytic transfusion reaction better than Alb-IAT among Japanese transfusion recipients in this retrospective survey of limited power.
Key words: indirect antiglobulin test, delayed hemolytic transfusion reaction, red cell antibody, polyethylene glycol
Key words: indirect antiglobulin te
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